Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Western Blotting Analysis: A representative lot detected exogenously expressed murine and rat CD1d in lysates of transfected Raji cells (Monzon-Casanova, E., et al. (2010). PLoS One.;5(9): e13089).
Flow Cytometry Analysis: A representative lot detected the surface expression of CD1d on isolated thymocytes from rats and mice, as well as on Raji transfectants expressing murine or rat CD1d (Monzon-Casanova, E., et al. (2010). PLoS One. 5(9): e13089).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD1d from isolated mouse and rat thymocytes, as well as exogenously expressed murine and rat CD1d from transfected Raji cells (Monzon-Casanova, E., et al. (2010). PLoS One. 5(9): e13089).
Activity Assay: A representative lot inhibited CD1d antigen presentation cells from activating type I NK cells using total splenocytes from rats and mice. Clone WTH-2 also blocked mCD1d1-transduced LBB cells from activating co-cultured VIII24.1 murine type II NKT cells, as well as mCD1d1-transduced Raji cells from activating co-cultured XV19.2 T murine type II NKT cells (Monzon-Casanova, E., et al. (2010). PLoS One. 5(9): e13089).

This Anti-CD1d Antibody, clone WTH-2 is validated for use in Flow Cytometry, Western Blotting, Immunoprecipitation, and Activity Assay for the detection of CD1d.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD1D antigen, CD1d1 antigen, T-cell surface glycoprotein CD1d) is encoded by the Cd1d (also known as Cd1d1) gene (Gene ID 25109) in rat species. CD1d is a nonpolymorphic MHC class I-like molecule which presents nonpeptide ligands (e.g. glycolipids) to natrual killer T (NKT) cells. NKT cells are known to have multiple effects on innate and adaptive immune responses and on the development of pathological conditions. In additon to hematopoietic cells, CD1d protein is also reported to be detected in non-lymphatic tissues in rat, such as the exocrine pancreas and Paneth cells. CD1d is initially produced with a signal peptide sequence (a.a. 1-17), the removal of which yields the mature CD1d with a large extracellular region (a.a.18-304), a transmembrane domain (a.a. 305-325), and a short cytoplasmic tail (a.a. 326-336).

Immunogen

Epitope: extracellular domain

Rat CD1d-transduced M12.4.1.C3 cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives.

Protein G Purified

Format: Purified

Quality

Evaluated by Flow Cytometry in rat thymocytes and splenocytes.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD1d in rat thymocytes and splenocytes.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~38 kDa calculated